Fig. 1.

Effect of VPA on malignant glioma cells. (A) Cytotoxic effect of VPA on U87MG, SF295, and T98G glioma cells for 96 h. The viability of the untreated cells was regarded as 100%. Points, mean of three independent experiments; bars, SD. (B) Effect of VPA on cell cycle in three glioma cell lines. After serum deprivation for 36 h, malignant glioma cells treated with or without VPA (1.0 mM) for 96 h were collected and stained with propidium iodide and analyzed in the FACScan. The percentage of cells in different phases of the cell cycle was determined using Cell Quest software.