Fig. 8.

VPA induces autophagy-related death in U87MG intracerebellar xenografts.

Mice bearing intracerebellar xenografts were killed at the end of drug treatment and compared with the untreated group. (A) The number of cells identified by immunohistochemical staining with MAP1-LC3 were counted in digitally captured images under high-power (10 × 40) magnification and graphed as the percentage of total cells. Columns, mean; bars, SE. *P < .05, as compared to the PBS group (n = 4); **P < .05, when compared with single drug (n = 4). (B) Induction of autophagy in necrotic cells after drug treatment. Necrotic cells were stained with MAP1-LC3 antibody. Black arrow head indicates the site of necrotic cells with MAP1-LC3 staining. Bar, 100 µm.