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. 2010 Jan 27;12(4):389–400. doi: 10.1093/neuonc/nop046

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© The Author(s) 2010. Published by Oxford University Press on behalf of the Society for Neuro-Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Inhibition of p53 diminishes apoptotic response to chloroquine in glioma cell lines with wtp53. (A) Assessment of the efficacy of endogenous p53 inhibition by transfection with unspecific scr-siRNA or p53-siRNA. U251 cells, which express high levels of endogenous mutp53 were transfected with scr-siRNA or p53-siRNA and stained with antibody DO-7 to ascertain the effect of siRNAs. (B) p53 inhibition by p53-siRNA diminished significantly (P < .02) the activation of caspase-3 in glioma cell lines with wtp53 but not in mutp53 lines. The percentage of cells with activated caspase-3 was determined by counting at least 500 cells in 5–10 microscopic fields from three replicate cover slips. The results shown represent the mean of two experiments.