Fig. 7.
Analysis of ATRT and GBM for MGMT methylation and expression. (A) Results from methylation-specific PCR show a lack of methylated MGMT promoter in ATRT DNAs (BT-12, BT-16, and 7368 xenograft), whereas DNA from GBM GS-2 shows a PCR product when using methylation-specific primers (M; U denotes reaction product when using primers for unmethylated MGMT promoter). (B) MGMT immunoblot analysis of protein extracts from ATRT cell lines (BT-12 and BT-16), and from paired patient tumor–primary xenograft protein extracts (7368pt and 7368x, respectively). Results show readily detectable MGMT protein in all ATRT specimens, whereas no MGMT protein is detected in the extract from GS-2 cells.