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. 2009 Dec 22;12(4):351–365. doi: 10.1093/neuonc/nop023

Fig. 4.

Fig. 4.

Developing an immunosuppressive phenotype in U251-conditioned monocytes in vitro depends on direct monocyte/glioma cell contact. (A) B7-H1 expression by flow cytometry in naïve (unconditioned) monocytes, monocytes cultured directly with U251 (contact), and monocytes cultured with U251, but separated by a membrane with 0.1 µm pores (noncontact). Both contact and noncontact CM had increased B7-H1 expression compared with unconditioned monocytes (*P < .05), but contact CM had significantly more B7-H1 expression than noncontact-CM (+, *, P < .001). (B) Increased T-cell apoptosis by flow cytometry (CD3+/AnV+/7AAD+) over background (not shown) in activated T cells cultured with U251-CM (contact or noncontact). Only U251-contact CM significantly increase activated T-cell apoptosis over background (*P < .05). (C) E. coli cell wall particle phagocytosis in naïve (unconditioned), contact-U251-conditioned, and noncontact-U251-CM. Contact-U251-conditioned phagocytosis is significantly decreased (*P < .05) compared with unconditioned or noncontact-U251 CM. Graphs are mean ± SEM of three separate experiments using different PBMC donors.