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. 2009 Dec 22;12(4):351–365. doi: 10.1093/neuonc/nop023

Fig. 5.

Fig. 5.

MDSC are increased in glioma patients' blood and GCM in vitro. (A) Representative dot plots and histograms showing strategy for determining MDSC frequency in peripheral blood. After gating on monocytes/granulocytes by forward and side scatter, lineage negative/HLA-DR−/CD33+ MDSC were identified in patients and normal donors. (B) Glioma patients had increased MDSC (expressed as a percentage of Lin− cells) compared with normal donors. (C) Representative dot plots demonstrating CD33 and HLA-DR expression patterns in lineage-negative monocytic/granulocytic cells from among NM and U251-CM. The glioma (U251)-CM have consistently increased CD33+/HLA-DR−/Lin− MDSC (n = 3).