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. 2009 Dec 22;12(4):351–365. doi: 10.1093/neuonc/nop023

Fig. 7.

Fig. 7.

True granulocytes do not contribute to GCM or MDSC although both are enriched for small granular cells. (A) Representative dot plots from 1 of 3 separate experiments performing 3 color flow cytometry for NM and U251-CM. CD11b expression is constant while CD14 expression is reduced (particularly for U251-CM). CD15 expression is absent, ruling out a contribution of granulocytes to our GCM. (B) U251-CM are enriched for small, relatively granular cells (low-forward scatter, relatively high-side scatter). Representative dot plots and pooled data from 3 experiments are shown. (C) MDSC from normal donors and (particularly) glioblastoma patients are also enriched for relatively small, granular cells (low-forward scatter, high-side scatter). Representative dot plots (corresponding to the normal donor and patient shown in Fig. 5A) and pooled data from all patients and donors shown in Fig. 5B. (D) Scatter plot showing increased MDSC in glioblastoma patients compared with normal donors using a lineage cocktail (CD3, CD14, CD16, CD19, CD20, CD56) that excludes granulocytes (CD16+) in the lineage negative fraction. This rules out a contribution of granulocytes to the MDSC we have identified.