FIG. 4.

Levels of transduction-capable rAAV correlate well with the increased levels of packaged genomes. Shown are the results of a transduction assay of HeLa cells, plated on 24-well dishes 24 hr before infection with 10-fold serial dilutions of rAAV8 (5 × 10⁷ packaged genomes per well). GFP-positive cells were counted 48 hr postinfection. Top left: Phase-contrast microscopy. Bottom left: GFP-positive cells. Right: The number of packaged genomes per well was divided by the approximate number of GFP-positive transduced cells to obtain the efficiency of infection. Values indicated are expressed as a ratio of packaged genomes per transducing unit. The ssDNA/TU ratio for all rAAVs tested in the absence of Ad ranged from approximately 1400 to 2000. Data are from three experiments and show standard deviations.