Table 1.
KY895 transformed with | LD100 for AZT (μM) |
---|---|
pGEX-2T | >100 |
toTK1 | 0.0316 |
HSV-tk | 1.000 |
toTK1 or HSV-tk was subcloned into pGEX-2T, and the TK-deficient E. coli strain KY895 was transformed with the resulting plasmids or the empty vector. Growth of colonies was tested with logarithmic dilutions of AZT. The values are the average from 2 to 4 independent experiments.