Table 2.
Substrate | Km (μM) | Vmax (mU/mg) | kcat (per s) | kcat/Km (per M per s) |
---|---|---|---|---|
Thymidine | 4.4 | 739 | 0.32 | 72 707 |
Azidothymidine | 3.1 | 605 | 0.26 | 84 485 |
Thymidine monophosphate | 16.03 | 0.23 | 9.96 × 10–5 | 6.2 |
Azidothymidine monophosphate | 26.21 | 0.074 | 3.2 × 10–5 | 1.2 |
Each shown result is an average of 2 independent experiments and the observed variation was less than 10%.