Table 2.
Kinetic parameters and substrate specificity of recombinant toTK1 demonstrate that this enzyme is a thymidine/azidothymidine and thymidine/azidothymidine monophosphate kinase
| Substrate | Km (μM) | Vmax (mU/mg) | kcat (per s) | kcat/Km (per M per s) |
|---|---|---|---|---|
| Thymidine | 4.4 | 739 | 0.32 | 72 707 |
| Azidothymidine | 3.1 | 605 | 0.26 | 84 485 |
| Thymidine monophosphate | 16.03 | 0.23 | 9.96 × 10–5 | 6.2 |
| Azidothymidine monophosphate | 26.21 | 0.074 | 3.2 × 10–5 | 1.2 |
Each shown result is an average of 2 independent experiments and the observed variation was less than 10%.