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. 2010 Feb 14;12(6):528–539. doi: 10.1093/neuonc/nop060

Fig. 3.

Fig. 3.

Ezrin overexpression promotes cell growth in NF2-positive GBM cells. (A) Proliferation analysis of Myc-tagged ezrin stably expressed in the NF2-positive LN229 and LN18 cells. (B) NF2 silencing by shRNA (sh375) in LN229 cells increased cell proliferation compared with vector control cells (Vect). The Western blot and quantification analyses show the depleted NF2 levels normalized to actin levels, in comparison to vector control levels. (C) Western blot and proliferation analyses of LN229 cells with combinatorial infections of Myc-tagged ezrin, NF2 shRNA-375 (sh), and their corresponding vectors (V), showing no effect of ezrin overexpression on the cells depleted of NF2. Statistically significant differences are indicated with asterisk. (D) GST pull-down assay of lysates from LN229 cells overexpressing ezrin (Ez) or vector (V) with PAK-RBD domain that precipitates active GTP-bound Rac1. Rac 1 fold activation was quantified by normalizing activated Rac1 levels to GST-PAK-RBD input. TL, total lysate.