Fig. 4.
Characteristics of the ependymoma cell line BXD-1425EPN. (A) Morphology of cells with 50% (left panel) and 100% (right panel) confluence. (B) Representative images of IMF staining. Cells were processed for immunofluorescent labeling to detect the presence of MT, Notch1, Nestin, GFAP, and MAP2. Nuclei were counterstained with DAPI. (C) Log-rank analysis of animal survival times of the mice injected with different amount of cells, ranging from 1 × 104 (10K) to 1 × 105(100K), from passage 10 (p10) and passage 68 (p68) into right cereberum. (D) H&E staining of orthotopic xenograft tumors generated from passage 10 (p10) and passage 68 cells (a and c, ×20; b and d, ×40).