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TGF-beta1 down-modulates CX3CL1 expression in primary glioma cell cultures. (A) TGF-beta1 levels were evaluated in the supernatants of glioma cells after 18 hours culture by ELISA assay. Histograms represent the mean ± SD of at least 3 independent experiments performed. (B) CX3CL1 mRNA was analyzed by RT–PCR on 3 primary glioma cells following 18 hours culture with or without (–) recombinant human TGF-beta1 (10 ng/mL) at 37°C, 5% CO2. Data are expressed as in Fig. 5B.
