Fig. 5.
Secretion of monocyte-affecting cytokines and growth factors are altered by tumor derived factors and DEX. (A) Plasma samples of peripheral whole blood were analyzed for cytokines and growth factors using the 22-Plex (Upstate) kit or ELISA. Cytokines with significant differences are shown. Normals donors are represented in filled circles, GBM patients in open circles, and GBM patients receiving DEX in open triangles. For RANTES samples, all GBM patients were grouped together. (B) Analysis of cell culture supernatants from primary and established GBM cell lines grown in the presence or absence in DEX. Each point to point connection represents paired samples prior to or after treatment with 0.1 µM DEX for 72 hours. One primary GBM cell line and one established GBM cell line, U373, were also subjected to various doses of DEX treatment. A representative experiment is shown. (C) Correlation between DEX dose, CD14+HLA-DRlo/neg monocytes, and plasma CCL2. * indicates significant difference (P < .05).