Fig. 2.

SPARC and PTEN expression and morphology for the generated U87MG-transfected cell lines. (A) Control (C2a2, C1.1) and SPARC-expressing (A2b2, H2) cells transfected with PTEN or EV were lysed 48 h after plating on fibronectin. Lysates (15 µg) were analyzed for PTEN and SPARC/SPARC-GFP expression by Western blot. wt, wildtype. Top arrow: transfected WT PTEN. Bottom arrow: endogenous mutant PTEN. (B) Cell morphology was assessed after equal numbers of the cells were plated for 5 days. Images were taken at ×10 (upper panels) and ×20 (lower panels) magnification. Inset shows a 1.6-fold zoomed image of cells.