Fig. 4.

NVP-AEW541 is active in combination with PI3K or mTOR inhibitors in cultures with an activating PIK3CA mutation. Cell cultures 21 and 34 were treated with or without 1 µM NVP-AEW541, 20 µM PI3K inhibitor LY294002, or 1 nM mTOR inhibitor rapamycin. Growth inhibition was quantified by comparing final cell number in control-treated cultures, which was set to 1, with cell number in drug-treated cultures. The extent of growth inhibition was determined after 4 days of culture. Results are derived from 3 experiments. Error bars indicate the standard deviation. P values were determined by Student's t-test.