HDMX is amplified in primary GBMs. (A) A tag density map from digital karyotyping of GBM sample D456 revealed subchromosomal changes. For all chromosomes (labeled 1–22, x, and y), Y-axis values indicate genome copies per haploid genome, while X-axis values represent positions along the chromosome, in Mb. Prominent large-scale genomic amplifications, including chromosome 1q32, 7p11, and 8q24, are clearly observed. (B) Genomic amplifications encompassing HDMX in chromosome 1q32 in 3 GBMs. (C) Genome-wide copy-number measurements of genomic DNA from one GBM xenograft by using Illumina HumanHap550 Genotyping BeadChip array. Three prominent, large-scale genomic amplifications are observed at 1q32, 7p11, and 8q24. The red arrows point to the 3 distinguishing regions of amplification. (D) A drill-down map of the SNPs displays high copy-number gain of multiple SNPs in a region of chromosome 1q32 containing HDMX.