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. Author manuscript; available in PMC: 2011 Sep 3.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Aug 4;399(4):623–628. doi: 10.1016/j.bbrc.2010.07.127

Figure 3. The N-terminal Ski is responsible for the stabilization effects on HDAC3 and RARα.

Figure 3

(A) COS-1 cells were transfected with Flag-RARα alone or co-transfected with Ski (wild type, N-terminal and C-terminal truncation mutants) and Flag-RARα as indicated. After 24 hrs transfection, cells were treated with CHX and RA for different times as shown in the figure and Flag-RARα levels were determined and compared by western blot analysis using an anti-Flag antibody. (B) The HDAC3 levels were detected using similar methods described in (A) under conditions indicated in the figure. (C) Schematic summary of the relationship between the Ski-Siah2 interaction and the ability of Ski to stabilize HDAC3 and RARα.