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. 2010 Sep 16;6(9):e1001107. doi: 10.1371/journal.ppat.1001107

Figure 5. The NEDD4-2s residual C2 domain is sufficient to target some isolated HECT domains to Gag.

Figure 5

(A) Residual C2 domain/HECT domain fusion proteins differ in their ability to induce the ubiquitination of ZWT Gag, and to associate with ZWT VLP. (B) The ubiquitination of ZWT Gag by the isolated HECT domain of HECW2 and its association with ZWT VLP were dependent on the presence of the NEDD4-2s residual C2 domain. 293T cells were transfected with the ZWT Gag construct (2 µg) and empty vector, or vectors expressing FLAG-tagged versions of NEDD4-2s (2 µg) or of the indicated HECT domain constructs (6 µg each).