Figure 4. The hk1 hk2 double mutant and the cheA1 or cheA2 mutant have normal level of Rrp2 activation.
Various strains of spirochetes (labeled on the top) were grown at 35°C, harvested in the late logarithmic phase (5×107 spirochetes/ml), and subjected to immunoblot analysis using monoclonal antibodies specific against Hk2, OspC or FlaB as indicated.
