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. 2010 Sep 16;6(9):e1001104. doi: 10.1371/journal.ppat.1001104

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Schematic diagram of predicted Rrp2 domain structure and various versions of overexpressed N-terminal receiver domains. D52 is the putative phosphorylation site. (B) Immunoblot of wild-type strain (lane 1), the strain carrying the shuttle vector only (lane 2), the strain with overexpression of Rrp2-N (lane 3), the strain with overexpression of Rrp2-N(D52A) (lane 4), and the strain with overexpression of Rrp2-N(D52E) (lane 5). Cultures were grown to late logarithmic phase at 35°C. Pooled antibodies/antisera against Rrp2, FlaB, and OspC were used. Bands corresponding to each protein were labeled on the right. (C) qRT-PCR analysis of ospC expression in various strains shown in (B). Levels of ospC transcript were normalized per 1000 copies of flaB in each sample.