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EGF enhances nuclear and cytoplasmic survivin protein levels. INS-1 cells were serum-deprived overnight then treated with or without EGF for 4 hours. (A) Cells were fixed and immunostained with a survivin antibody, stained with DAPI (DNA detection) then analyzed by Z-stack confocal microscopy. (B) Nuclear and cytoplasmic protein fractions were prepared, resolved by SDS-PAGE then transferred to PVDF membranes and immunoblotted with anti-survivin, anti-actin, and anti-HDAC1.
