Figure 2.

Genetic labeling of adult OPCs in the piriform cortex. A, Almost all EYFP⁺ cells expressed Sox10 (red). B, C, A subpopulation of EYFP⁺ cells were NG2⁺ (red) or PDGFRα⁺ (red) putative OPCs, respectively. D, Recombination rate in NG2⁺/PDGFRα⁺ OPCs on different days after last tamoxifen injection. Note that recombination reached a plateau after 5 d after TM. E, Recombination rates in NG2⁺/PDGFRα⁺ OPCs at different ages. Virtually no NeuN⁺ (red) (F) or HuCD⁺ (red) (G) cells were labeled before 17 d after TM. H, No Iba-1⁺ (red) microglia were labeled with EYFP. Arrows in A–C point to examples of double-labeled cells. Arrowhead in A, single-labeled cell. Scale bars, 50 μm.