Figure 6.

OPCs generate immature neurons. A–A3, Triple-fluorescent confocal z-sections show an EYFP and low-level DCX (red) double-positive perineuronal progenitor that is adjacent to an HuCD⁺ (blue) neuron. B–B3, Arrows designating an immature neuron that expresses EYFP, HuCD (blue), and high-level DCX (red), with arrowheads and insets showing an example of mature neurons that display EYFP and HuCD (blue) expression and undetectable DCX (red). Cells pointed by arrowheads in B–B3 are shown as orthogonal views in corresponding insets. C–C3, Immature EYFP⁺ neurons are colabeled with Tuj1 (red) in both cell bodies (arrowheads) and neurites (arrows) in this single confocal slice. D–D3, EYFP⁺/HuCD⁺ (blue) neurons (arrows) show nuclear Tbr1 immunoreactivity (red). D1–D3, Single-channel view of image D. All pictures are taken from piriform cortex. Scale bars, 10 μm.