Figure 7.

Morphology and phenotype of OPC-derived neurons. A, An EYFP⁺ neuron in layer II of piriform cortex displays typical morphology of pyramidal neurons. A single thick apical dendritic trunk (arrow) extending toward pial surface (top left) and branching at layer I (wavy arrow). Multiple basal dendrites (arrowheads) extending toward layers II and III. Inset in A shows EYFP and NeuN double immunostaining. B, C, EYFP fluorescence micrographs showing layer II pyramidal neurons with varied morphologies, including different lengths of apical dendritic trunks (arrows in B, C). Insets in B and C shows EYFP⁺/NeuN⁺ (red) cell bodies. D, An example of layer III EYFP⁺ large pyramidal neurons. E, Orthogonal view of EYFP and vGLUT1 (red) double-immunostaining micrograph. Note punctate vGLUT1 immunoreactivity in EYFP⁺ neuron (arrows). F, G, Large-sized EYFP⁺ neurons (arrows) were negative for GAD67 (red) and ChAT (red), respectively. Arrowheads in F point to GAD67⁺ cells. Inset in G shows a positive control of ChAT immunostaining from striatum area (Str). Scale bars: A–D, E, G, 10 μm; F, 50 μm.