Table 2.
Percentage of PFPA–Thiol 3 |
d-Mannose Density [nmol mg−1 Au NPs] |
Surface Coverage [%] [a] |
Kd [nM] |
---|---|---|---|
10% | 2.0 ± 0.54 | 2.8 | 123 ± 16 |
30% | 5.3 ± 0.83 | 7.4 | 21.4 ± 7.3 |
50% | 11.2 ± 2.32 | 16 | 16.3 ± 5.4 |
70% | 24.3 ± 2.78 | 34 | 14.4 ± 2.4 |
90% | 35.2 ± 1.29 | 49 | 12.7 ± 4.0 |
95% | 42.6 ± 3.81 | 59 | 9.4 ± 2.3 |
98% | 50.3 ± 4.17 | 70 | 6.7 ± 1.4 |
100% | 57.4 ± 3.21 | 80 | 5.3 ± 1.6 |
The surface coverage was defined as the percentage of ligands coupled versus the theoretical maximal number of ligands occupying the nanoparticle [61].