Table 3.
Anti-tuberculosis and cytotoxic activities, and resulting selectivity indices (SI) of Micromelum constituents 1 – 7
| Material/compound | MIC[μg/ml]a | Cytotoxicity IC50 [μg/ml]b | SIc | Macrophage assayd (μg/ml) | ||
|---|---|---|---|---|---|---|
| VERO | J774 | EC90 | EC99 | EC99 | ||
| CHCl3 extract | 12.5 | |||||
| 1 | 1.5 ± 0.4 | 95 | 40.71 | 63 | 5.6 | 18.9 |
| 2 | 31.5 ± 0.2 | > 102 | >3 | |||
| 3 | 14.3 ± 0.9 | > 102 | >7 | |||
| 4 | > 128 | > 102 | ||||
| 5 | > 128 | > 102 | ||||
| 6 | 42.3 ± 0.5 | 101 | ||||
| 7 | 15.6 ± 0.2 | > 102 | >7 | |||
| Rifampine | 0.040 ± 0.017 | 100 | 2500 | 0.05 | 0.18 | |
| Streptomycin | 0.1 | 0.3 | ||||
a
Minimum inhibitory concentration (MIC90) values against M. tuberculosis (H37Rv) in the MABA assay.
b
Medium inhibitory concentration (IC50) on Vero cells.
c
Selectivity Index (SI) calculated by the quotient of the VERO IC50 and the MIC90.
d
Concentration effecting a reduction of M. tuberculosis cfu of 90% (EC90) or 99% relative to untreated control.
e
Positive control; negative control was solvent.