Figure 5.

Linear antibody epitope mapping. PLL-ELISA was used to detect the responses of the antibody against each linear peptide. The concentration of each peptide of HIVCN54gp145 was 10 μg/ml. In these epitopes, seven were defined as positive (> two-fold background). Epitopes p4838, p4859 and p4860 were only identified in the SV145 group. P4876 was only defined in the SV145-10 M group. The grey line in the figure indicates the threshold value (0.22), which is two times than the average OD values (0.11) from mock controls. Five mutation regions were labeled.