Table 1.

List of the primers used in PCR for modification

Name	Primer sequence (5'-3')
CN54145F	GCTCTAGAGATATCGACACCATGGACAGGGCCAAGCTGCTGCTG
CN54145R	GTGAACAGGGTGAGGCAGGGCTACTGAGGATCCGTCGACCG
145M1u	ACCACCGAGTTCTGCGCCAGCGACG
145M1d	CGCAGAACTCGGTGGTGGTGGCGCCCTTCCACACGG
145M2u	AACCAGGACACCTACCACGAGACC
145M2d	CTCCTCGTGGTAGGTGTCCTGGTTGCTGCTCACGTTCCT
145M3u	ACCGTGGTGGAGGACAGGAAGCAGAC
145M3d	TTCCTGTCCTCCACCACGGTGGTGGCGTTG
145M4u	CTACGAGAAGAACAGCCAGGAGTACTACAGGCTGATC
145M4d	CCTGGCTGTTCTTCTCGTAGTTCTTCTTGGT
145M5u	ATCTTCAACCGCACCCAGCCCTGCTACAACGTGAGCACCG
145M5d	GTTGTAGCAGGGCTGGGTGCGGTTGAAGATCTTGTC

All mutations in primers are marked with bold text.