Figure 2. Luciferase reporter assays.

(A) Comparison of the two alleles of marker GF100472 with the extreme sizes [(CA)₈ and (CA)₁₅] and (B) with either of GF100472 alleles (CA)₈, (CA)₉, (CA)₁₀, (CA)₁₁ and (CA)₁₅. pGL2 vector containing the C3 promoter region with different CA-repeat variants [(CA)₈, (CA)₉, (CA)₁₀, (CA)₁₁ and (CA)₁₅] was cotransfected into HEK293T cells with a β-galactosidase expression vector (pHSV-LacZ) for normalization of transfection efficiencies. In parallel, empty (promoterless) vector (pGL2-basic) was transfected with pHSV-LacZ. Transcriptional activities were determined by quantifying the luciferase activity of cellular extracts prepared 48 h after transfection. Relative luciferase activity of (CA)₈ was defined as 100. Data show the mean ± SD relative activity from three experiments done in triplicate. Statistical significances were determined by two-tailed unpaired t test. (**) indicates p<0.001. (*) indicates p<0.05. (NS) indicates non significant.