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. Author manuscript; available in PMC: 2011 Sep 15.
Published in final edited form as: Cancer Res. 2010 Aug 31;70(18):7294–7303. doi: 10.1158/0008-5472.CAN-09-3982

Figure 1. Analyses of spheroids formed from putative normal and cancer stem cells.

Figure 1

A. Illustration of the placement of an insert (pore size: 8.0 μm) containing stromal cells on top of the Matrigel for the co-culture experiments (E: epithelial cells; S: stromal cells). B. Phase contrast images of representative spheroids formed from either N-LS or T-LS cells after 14 days of co-culture with primary stromal cells from different sources. Bar, 100 μm. C. Illustration of the different sizes of the spheroids formed from tumor LSC cells after co-culturing with either NPFs or CAFs. Bar, 100 μm. D. Comparative immunohistochemical analysis of spheroids formed from normal and tumor LS cells co-cultured with UGSM, NPFs or CAFs. Sections of spheroids were analyzed by co-immunofluorescence using antibodies against the basal cell marker p63 (red) and luminal cell marker CK8 (green). DAPI was used for labeling cell nuclei. Bar, 25 μm.