Skip to main content
. Author manuscript; available in PMC: 2011 Sep 15.
Published in final edited form as: Cancer Res. 2010 Jul 28;70(18):7176–7186. doi: 10.1158/0008-5472.CAN-10-0697

Figure 1.

Figure 1

miR-16 suppresses Wip1 expression by targeting WIP1 3’UTR. (A) Predicted miRNA sequences and their putative recognition sites within 3’ UTR of WIP1. (B) Wip1 expression is suppressed by ectopic miR-16 transfection. U2OS cells were transfected with the indicated miRNAs. 72 h after transfection, Wip1 protein levels in each sample were detected by immunoblotting (upper panel), quantitated according to the intensity of Wip1 bands, and normalized with the control sample (bottom panel). (C) Immunoblots of endogenous Wip1 in U2OS cells transfected with control miRNA, miR-16 or antagomir-16, and treated with 500 ng/ml NCS at 72 h after transfection. (D) MiR-16 specifically targets the 3’ UTR of WIP1. A luciferase construct containing wildtype WIP1 3’UTR (left panel) or mutant WIP1 3’ UTR (right panel) was transfected into U2OS cells with the indicated miRNAs or antagomirs. Renilla luciferase activities were measured 48 h after transfection and normalized to firefly luciferase. Relative luciferase activity (luminescence) was obtained after normalizing with the control number.