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. Author manuscript; available in PMC: 2011 Sep 15.
Published in final edited form as: Cancer Res. 2010 Jul 28;70(18):7176–7186. doi: 10.1158/0008-5472.CAN-10-0697

Figure 3.

Figure 3

MiR-16 suppresses cell proliferation and sensitizes MCF-7 cells to doxorubicin. (A) Growth curves of Wip1+/+ and Wip1−/− MEFs infected with control or miR-16 expressing pseudoviruses. (B) MiR-16 inhibits the expression of Wip1 in MCF-7 cells. MCF-7 cells were transfected with control or miR-16 expression vector DNA. 36 h after transfection, cell lysates were harvested or total RNA was extracted for the analyses of miRNAs (by quantitative PCR, left panel) and proteins (by Western blotting, right panel). (C) MiR-16 sensitizes MCF-7 cells to doxorubicin. MFC-7 cells were transfected with control, Wip1 shRNA, miR-16 expression vector DNA, or miR-16 and Wip1 shRNA expression vector DNA. 48 h after transfection, cells were incubated with increasing doses of doxorubicin as indicated. Cell viability was determined 3 days after incubation.