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. Author manuscript; available in PMC: 2011 Sep 15.
Published in final edited form as: Cancer Res. 2010 Jul 28;70(18):7176–7186. doi: 10.1158/0008-5472.CAN-10-0697

Figure 4.

Figure 4

MiR-16 is downregulated in mammary tumor stem cells. (A) Levels of Wip1 and miR-16 in mouse mammary tumors are not significantly different from those of normal mammary tissues. N1-3: normal mammary gland tissues; T1-3: mammary tumors. Levels of Wip1 mRNA and miR-16 were determined by quantitative RT-PCR. (B) Micrographs of mammospheres originated from mammary tumor stem cells. TC-1, 2: mammary tumor cells; MS-1, 2: mammospheres from the corresponding mammary tumor cells. (C) Expression levels of stem cell markers and differentiation markers in mammospheres and their original tumors. Stem cell markers (Oct-4, KLF-4), differentiation markers (Keratin-14 and Keratin-18), miR-16, and β-actin (as control) were analyzed by semi-quantitative RT-PCR in starting tumor cells and mammospheres. NC: normal tissue cells. (D) miR-16 is upregulated in cells differentiated from mammospheres. Wip1 protein levels were increased in mammospheres, but were downregulated when mammospheres were differentiated to non-stem cells (Rec: recovered cells) (upper panels). In contrast, miR-16 was downregulated in each of the individual mammospheres, but was upregulated in re-differentiated cells. Levels of miR-16 were determined by quantitative RT-PCR (bottom panel).