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. Author manuscript; available in PMC: 2011 Sep 15.
Published in final edited form as: Cancer Res. 2010 Jul 28;70(18):7176–7186. doi: 10.1158/0008-5472.CAN-10-0697

Figure 5.

Figure 5

MiR-16 inhibits the maintenance and proliferation of mammary tumor stem cells. (A) miR-16 and the Wip1 inhibitor reduce the number of mammary tumor stem cells. Cells isolated from mammary tumors in transgenic MMTV-erbB2 mice were infected by control viruses or pseudoviruses expressing miR-21, miR-16, and/or mouse Wip1, and cultured in the mammosphere-forming medium with or without 5 μM of the Wip1 inhibitor CCT007093. 10 days after incubation, mammospheres were observed and their sizes and numbers were determined. (B) Mammosphere formation is inhibited by miR-16, the Wip1 inhibitor, or Wip1 shRNA, and restoration of Wip1 reversed the effects of miR-16 on blocking mammosphere formation in culture. Mammosphere numbers (left panel) and sizes (right panel) were measured under each condition.