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. Author manuscript; available in PMC: 2011 Mar 16.
Published in final edited form as: Oncogene. 2010 Jun 21;29(37):5159–5170. doi: 10.1038/onc.2010.255

Figure 3.

Figure 3

Transient re-expression of CXCL14 induces cell death. Cell survival was evaluated by flow cytometry using a GFP containing expression vector. The normal level of cell death which occurs in 4-5% of H23 cells (a) was increased to 15-20% due to exposure to the transfection reagent alone (data not shown). GFP expression in the transfected cells increased cell death by 20-25% which was only marginally greater than seen with the transfection media (b). In contrast, expression of CXCL14-GFP led to a 40-45% cell death (c). This indicates that re-expression of CXCL14 in H23 cells where the endogenous expression is silenced by methylation increased cell death by 19.3 % compared to the GFP control (d).