Table 1.
Promoter methylation of CXCL family genes in lung cancer
| No. | Gene | CpG island property |
Methylated (%) |
Array** positive (#) | |||||
|---|---|---|---|---|---|---|---|---|---|
| CpG* (#) | Size (bp) | GC (%) | Obs/exp | Cell lines | NHBEC | PBMC | |||
| 1 | CXCL1 | 54 | 626 | 69.3 | 0.74 | 0 | - | - | 0 |
| 2 | CXCL2 | 62 | 623 | 69.2 | 0.84 | 0 | - | - | 1 |
| 3 | CXCL3 | 62 | 636 | 69.0 | 0.84 | 0 | - | - | 1 |
| 4 | CXCL4 (PF4) | 22 | 302 | 65.6 | 0.68 | 82 | 90 | 100 | 2 |
| 5 | CXCL5 | 26 | 216 | 70.8 | 0.69 | 65 | 0 | 15 | 3 |
| 6 | CXCL6 | 23 | 207 | 73.4 | 0.85 | 65 | 0 | 55 | 1 |
| 7 | CXCL7 (PPBP) | 0 | - | - | - | - | - | - | 0 |
| 8 | CXCL8 (IL-8) | 0 | - | - | - | - | - | - | 1 |
| 9 | CXCL9 | 0 | - | - | - | - | - | - | 0 |
| 10 | CXCL10 | 0 | - | - | - | - | - | - | 1 |
| 11 | CXCL11 | 0 | - | - | - | - | - | - | 3 |
| 12 | CXCL12 | 223 | 2677 | 60.9 | 0.90 | 63 | 0 | 0 | 1 |
| 13 | CXCL13 | 0 | - | - | - | - | - | - | 0 |
| 14 | CXCL14 | 82 | 803 | 68.0 | 0.90 | 59 | 5 | 10 | 2 |
| 15 | CXCL16 | 134 | 1372 | 69.2 | 0.82 | 0 | - | - | 1 |
| 16 | CXCL17 | 0 | - | - | - | - | - | - | 0 |
*
When a gene has no CpG island the CpG number was shown as 0.
**
Array positive indicates the number of lung adenocarcinoma cell lines (out of 6 cell lines) that after DAC treatment showed a 2-fold or higher increase in expression.