Table 1.
Probe and target sequences
| Probes | Sequences 5′ → 3′ | # MM |
|---|---|---|
| G | (T)10 TGT GAC CCG GTA AGT G | PM |
| A | (T)10 TGT GAC CCA GTA AGT G | 1 MM |
| TG | (T)10 TGT GAC CTG GTA AGT G | 1 MM |
| TA | (T)10 TGT GAC CTA GTA AGT G | 2 MM |
| PC | (T)10 TGG AGC TGC TGG CGT | |
| Targets | Sequences 5′ → 3′ | |
| Gc | CTC ACT TAC CGG GTC ACA CT | |
| PCc | ACG CCA GCA GCT CCA | |
PM, perfect match; MM, mismatch. Mismatches are represented in boldface. Note that, on the 5′ end, depending on the grafting procedure, either a pyrrole moiety or a thiol (SH(C)6) is present on the probes.