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Principle of LASR. (a) T-jump profile. Normalized emission time trajectory of a single fluorescent bead. The heating period is highlighted in purple. During heating, the bead's fluorescence emission intensity decreases and then recovers rapidly after the T-jump. Inset: Exponential fit to the fluorescence emission recovery. (b) T-jump calibration curve. Jump temperatures are controlled by ND filters. Temperature is calibrated with a micrometer-sized gold sensor.
