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. 2010 Sep 22;99(6):1925–1931. doi: 10.1016/j.bpj.2010.07.019

Figure 3.

Figure 3

Validation of LASR. UV melting curves (black) for DNA duplexes (a,b, and c, as indicated) and corresponding normalized single-molecule melting curves (red). Single-molecule melting curves are obtained by calculating the fraction of molecules that transition from low to high FRET after a LASR T-jump, and normalizing the high-temperature fraction to one. Error bars were calculated as 1/n, where n is the number of molecules observed.