Figure 1.

Spectra of HbS before and after dialysis through a membrane with a 2000 g mol⁻¹ molecular mass cutoff are compared to a spectrum calculated for a similar HbS concentration using data from Zjilstra et al. (70). HbS was converted to a CO-state before spectrophotometry characterization, and 50 mM of sodium dithionite were added. Because of the low HbS concentration, absorbance at wavelengths of λ > 600 nm is low and affected by noise.