Table 1.

Summary of tested combinations of HbS concentration and temperature of solutions for which there was no polymerization after dialysis

HbS concentration (mg ml−1)	Temperature (°C)	Duration of exposure to supersaturation (s)	θ in nondialyzed solution (s)
280	24.7	77.4	1.5
	27.7	77.4	0.7
237	22	77.4	8–38
	30.9	77.4	6–33
	31.0	128.7	6–33
	36.5	129.9	5–28
235	33	129.9	4–27
	35	129.9	3–22
	36.5	129.9	2–18

Dialysis was performed through a membrane with 2000 g mol⁻¹ molecular mass cutoff, and polymers did not appear after exposure to supersaturation for extended times. For comparison, the nucleation delay times, θ, from Galkin and Vekilov (31) are shown in the last column. Since aging of HbS solutions shortens θ, ranges of this variable are shown where data are available.