Figure 2.

CD spectra of M2 in DLPC (solid line) and POPC (dashed line) vesicles. The samples were prepared by premixing peptides and lipids in HFIP/chloroform, followed by solvent removal and hydration with 10 mM sodium phosphate buffer, pH 7, 500 mM NaCl. The samples were freeze-thawed three times and sonicated to achieve full equilibration. The peptide concentration in the vesicles was 2 mol %. The M2 peptides are helical in POPC and DLPC bilayers.