Figure 5.

(A) High-frequency elasticity modulus divided by the Rouse modulus G/G_R versus time t after the addition of topoisomerase II (4 units/μg) to a solution of 1.0 g of DNA/L without ATP (♢) or with ATP concentrations of 1.0 (□), 2.5 (●), or 4.0 (▵) mM. (B) As in panel A, but after the addition of 1 (▵), 2 (□), or 4 (●) units of topoisomerase II per μg of DNA to a solution of 1.0 g of DNA/L and 2.5 mM ATP. (For panels A and B: solid curves are guides to the eyes and dashed lines demarcate G/G_R = 1, i.e., the nonentangled state.)