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(A) mRNA analogs used in this work. (B) 80S ribosome complexed with mRNA analog (Pu, purine), tRNA, and translation termination factors. (C) Crystal structure of human eRF1. (D) Location of the NIKS and YxCxxxF loops within the N-domain (Song et al. 2000). The distance separating modified base and the first nitrogen of the azidogroup is ≤11 Å for U* and 14 Å for G*.
