FIGURE 5.
Contribution of large subunit rRNA components to tRNA selection. (A) The interactions between helix 69 (H69, teal) and a post-accomodation A-site tRNA on the ribosome are shown. The tip of H69 (residue A1913) is in close proximity to the D stem of the tRNA and the decoding center. tRNA residues highlighted (blue, green, or red) follow the same pattern as in Figure 4D. mRNA is represented in orange. E site (light pink) and P site (light brown) tRNAs are represented on the left for orientation. (B) Rates of accommodation (k5) for the formation of fMet-Trp dipeptide for WT, G24A, G59A, and 24/27/59 tRNATrp with WT and A1913U ribosomes on UGG (black bars) and UGA (gray bars) codons.