FIGURE 4.

In vitro regulation of splicing of RPL30 5A C9U by L30 in the absence of Cpb80. Synthetic RPL30 transcripts, indicated at the top, were incubated under splicing conditions with wt (A) and cbp80Δ (B) extracts, supplemented with MBP:L30 or MBP:Cbp80, as indicated. Upon completion of the reaction, RNA was extracted and analyzed by semiquantitative RT-PCR. Bands corresponding to substrate and spliced RNA are shown. Amounts of RNA, extracts, and recombinant protein were equivalent in all reactions.