Figure 6.

Effect of H- and L-chain siRNA on iron incorporation into ferritin. (A) Two and 4 days after transfection with either nontargeting control, FHsiRNA, or FLsiRNA, LECs were incubated for 20 hours with ⁵⁹Fe-transferrin. After cell lysis, proteins were precipitated with acetone, resuspended in PBS, an aliquot removed for protein determination and the remaining sample subjected to 8% native PAGE. The amount of ⁵⁹Fe in the ferritin band was normalized to total protein in each sample and data expressed as a percentage of that of control cells transfected with nontargeting siRNA. The histogram bars represent the mean of at least five samples. (B) A representative autoradiogram from the 4-day time point. *Significantly different from control (P < 0.05); **significantly different from control (P < 0.01); ANOVA and Tukey's test.