Figure 8.
Effect of ferritin H- and L-chain siRNA on cystine uptake and GSH concentration 4 days after transfection. LECs were transfected with either nontargeting control, FHsiRNA, or FLsiRNA, lysed, and intracellular GSH measured in the lysates. In a parallel set of experiments, l-35S-cystine uptake was then determined. Data are expressed as a percentage of control cells transfected with nontargeting siRNA. Histograms represent the mean ± SEM of at least four samples. *Significantly different from control (P < 0.01); ANOVA and Tukey's test.