Figure 9.

Effect of iron, iron chelation, FHsiRNA, and FLsiRNA on VEGF accumulation in LEC conditioned medium. LECs were transfected, and 4 days after transfection, VEGF was measured in the CCM. In a parallel set of experiments, nontransfected cells (LECs) were grown to confluence and treated with iron in the form of hemin (1.5 μM) or an iron chelator, dipyridyl (0.3 mM), in serum-free medium for 6 hours. VEGF accumulation in the CCM was determined. Data are expressed as a percentage of VEGF in the CCM of control cells for each respective experiment. *Significantly different from control, P < 0.05, ANOVA and Tukey's test.